Abstract: One hundred and twenty-four items were initially constructed based on promoting thinking rather than memorization and differentiating the well-known target people from the poorly known ones to knowledge about the zoonotic disease. The scores from sample respondents were subjected to item analysis, item selection comprising of item difficulty index, item discrimination index and point biserial correlation. Sixty-two items with a difficulty index ranging from 20 to 80 per cent; discrimination index ranging from 0.2 to 0.8 and items with significant point biserial correlation either at 1 per cent or 5 per cent level were included in the final format of the knowledge test. The reliability of the knowledge test was found to be 0.96 using split-half method which was highly significant. The knowledge test was developed and standardized which is highly stable and dependable for measurement.
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